MersV1, Main, Exploration, bibRecord, 005038

Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin

Identifieur interne : 005038 ( Main/Exploration ); précédent : 005037; suivant : 005039

Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin

Auteurs : Michio Ohba [États-Unis, Japon] ; Max D. Summers [États-Unis] ; Pepper Hoops [États-Unis] ; Gale E. Smith [États-Unis]

Source :

Journal of Invertebrate Pathology [ 0022-2011 ] ; 1977.

RBID : ISTEX:A7DD56A230FA83DA829B5C6B676D7EA7B31CE051

English descriptors

Teeft :
- Academic press, Acmnpv, Acmnpv loval, Acmnpv loval antiserum, Acmnpv polyhedrin, Antibody titers, Antiserum, Assay, Baculovirus, Baculoviruses, Bergold, Bombyx mori, Cell culture, Comparative studies, Etude comparee, Granulosis virus, Immunoradiometric assay, Inclusion body proteins, Individual wells, Insect pathol, Invertebrate tissue culture, Krywienczyk, Loval, Microtiter, Microtiter plates, Microtiter radioimmunoassay, Microtiter wells, Nonoccluded virus, Nuclear polyhedrosis virus, Nuclear polyhedrosis viruses, Nucleocapsids, Optimal conditions, Pathol, Phosphate buffer, Pieris brassicae, Polyhedrin, Polyhedrin antisera, Polyhedrosis, Preimmune serum, Radioimmunoassay, Serological, Serological characterization, Serological relationship, Serological relationships, Serological studies, Serum dilution, Similar experiments, Sodium azide, Specific activity, Specific antibodies, Viral, Viral antigens, Viral proteins, Volkman, Znvertebr.

Abstract

Abstract: A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. A preliminary study also demonstrated the ability to use this assay to detect specific antiviral antibodies.

Url:

https://api.istex.fr/ark:/67375/6H6-F4M5KS84-X/fulltext.pdf

DOI: 10.1016/0022-2011(77)90146-X

Affiliations:

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Le document en format XML

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<term>Baculovirus</term>
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<term>Individual wells</term>
<term>Insect pathol</term>
<term>Invertebrate tissue culture</term>
<term>Krywienczyk</term>
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<term>Microtiter wells</term>
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<front><div type="abstract" xml:lang="en">Abstract: A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. A preliminary study also demonstrated the ability to use this assay to detect specific antiviral antibodies.</div>
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Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin

Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri